9.5 The role of seed plants  (Page 2/16)

Scientific method connection

Testing attraction of flies by rotting flesh smell

Background: Visitation of flowers by pollinating flies is a function mostly of smell. Flies are attracted by rotting flesh and carrions. The putrid odor seems to be the major attractant. The polyamines putrescine and cadaverine, which are the products of protein breakdown after animal death, are the source of the pungent smell of decaying meat. Some plants strategically attract flies by synthesizing polyamines similar to those generated by decaying flesh and thereby attract carrion flies.

Flies seek out dead animals because they normally lay their eggs on them and their maggots feed on the decaying flesh. Interestingly, time of death can be determined by a forensic entomologist based on the stages and type of maggots recovered from cadavers.

Hypothesis: Because flies are drawn to other organisms based on smell and not sight, a flower that is normally attractive to bees because of its colors will attract flies if it is sprayed with polyamines similar to those generated by decaying flesh.

Test the hypothesis:

1. Select flowers usually pollinated by bees. White petunia may be good choice.
2. Divide the flowers into two groups, and while wearing eye protection and gloves, spray one group with a solution of either putrescine or cadaverine. (Putrescine dihydrochloride is typically available in 98 percent concentration; this can be diluted to approximately 50 percent for this experiment.)
3. Place the flowers in a location where flies are present, keeping the sprayed and unsprayed flowers separated.
4. Observe the movement of the flies for one hour. Record the number of visits to the flowers using a table similar to [link] . Given the rapid movement of flies, it may be beneficial to use a video camera to record the fly–flower interaction. Replay the video in slow motion to obtain an accurate record of the number of fly visits to the flowers.
5. Repeat the experiment four more times with the same species of flower, but using different specimens.
6. Repeat the entire experiment with a different type of flower that is normally pollinated by bees.

Analyze your data: Review the data you have recorded. Average the number of visits that flies made to sprayed flowers over the course of the five trials (on the first flower type) and compare and contrast them to the average number of visits that flies made to the unsprayed/control flowers. Can you draw any conclusions regarding the attraction of the flies to the sprayed flowers?

For the second flower type used, average the number of visits that flies made to sprayed flowers over the course of the five trials and compare and contrast them to the average number of visits that flies made to the unsprayed/control flowers. Can you draw any conclusions regarding the attraction of the flies to the sprayed flowers?

Compare and contrast the average number of visits that flies made to the two flower types. Can you draw any conclusions about whether the appearance of the flower had any impact on the attraction of flies? Did smell override any appearance differences, or were the flies attracted to one flower type more than another?

Form a conclusion: Do the results support the hypothesis? If not, how can this be explained?