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- Dna structure and function
- Dna structure and sequencing
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Card 7 / 9:
Provide a brief summary of the Sanger sequencing method.
The template DNA strand is mixed with a DNA polymerase, a primer, the 4 deoxynucleotides, and a limiting concentration of 4 dideoxynucleotides. DNA polymerase synthesizes a strand complementary to the template. Incorporation of ddNTPs at different locations results in DNA fragments that have terminated at every possible base in the template. These fragments are separated by gel electrophoresis and visualized by a laser detector to determine the sequence of bases.
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Questions & Answers
Explain the following terms .
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Source:
OpenStax, General biology i lecture. OpenStax CNX. Aug 25, 2015 Download for free at https://legacy.cnx.org/content/col11869/1.1
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